| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.011048 |
| Chromosome: | chromosome 10 |
| Location: | 5261853 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456400 | DAT1 | (1 of 1) PTHR11958//PTHR11958:SF63 - SODIUM/DICARBOXYLATE SYMPORTER-RELATED // EXCITATORY AMINO ACID TRANSPORTER 2, ISOFORM C; Dicarboxylate/amino acid cation sodium transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCATGCGCTGCGCCACCGAGGGGCTGGGCTGCGACCCGCGCATTGTGC |
| Internal bar code: | GGTCGAGGTCTGCGTATCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2206 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCCGAGATCAGAACCCG |
| Suggested primer 2: | CAAACGCCGCCCTGATTATG |