Insertion junction: CLIP2.011073_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.011073
Chromosome:chromosome 12
Location:1453029
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g488250 (1 of 2) K17822 - DCN1-like protein 1/2 (DCUN1D1_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTTGTGCATATCAGGAAACACGAGCACACTCATGTTTCTGTACTCCG
Internal bar code:TTGACCATAATCGCAACAGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3717
LEAP-Seq percent confirming:97.7477
LEAP-Seq n confirming:217
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:222

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGAAAGTTTTGGCGCCGA
Suggested primer 2:ACACACACACACACACACCT