Insertion junction: CLIP2.011081_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.011081
Chromosome:chromosome 12
Location:1826102
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g512500 (1 of 1) PF07962 - Replication Fork Protection Component Swi3 (Swi3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTGCCTGCTTCCAAACTGACCCTCCATGCTCGCGTGCGCACTGCATCC
Internal bar code:GTACACCACCATATCCCACGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1804
LEAP-Seq percent confirming:89.4737
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGGAATAGCACGTAGGC
Suggested primer 2:GTCTTGCTTGCAACCACAGG