| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.011084 |
| Chromosome: | chromosome 12 |
| Location: | 464313 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801344 | (1 of 5) PTHR19446:SF107 - INTRON MATURASE, TYPE II FAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGTGTCGACAGCAGCAGCAGCAGCTGTCTGCCATGCAGACATAGCCCT |
| Internal bar code: | GATCGTTCAAACTTCGACCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4212 |
| LEAP-Seq percent confirming: | 5.12821 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGTGTCATCCTCGGTTG |
| Suggested primer 2: | ACATGGAGGCCTTCTGCTTC |