Insertion junction: CLIP2.011121_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.011121
Chromosome:chromosome 11
Location:3944518
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g480200 (1 of 4) IPR006597 - Sel1-like repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTTTGTAGGACCACCCTGGAAGGCGCGACGTGGGAAGGATCGCTGACT
Internal bar code:CGATAACACGACTTCAAGATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1953
LEAP-Seq percent confirming:60.2041
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:98

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGCCTCATCATGTCCAAA
Suggested primer 2:TCTCTGTGTCCAAGTGCACC