| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.011124 |
| Chromosome: | chromosome 4 |
| Location: | 391067 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217912 | (1 of 1) PTHR13035//PTHR13035:SF0 - UNCHARACTERIZED // PROTEIN N-TERMINAL GLUTAMINE AMIDOHYDROLASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGGCTGGAGGAGTCAGGCGTGTAGCGCCCATAACACGCACATGCAC |
| Internal bar code: | GGAGCTCCACGTTGGCATGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 37.037 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACCTTGACACGGTACTG |
| Suggested primer 2: | TTCCACAATCAGTCTGCCCC |