Insertion junction: CLIP2.011133_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.011133
Chromosome:chromosome 2
Location:2966089
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCCTTCTTGTAACCAATGCAATCTGACGCGCATTGCTAGCGCTCTGGG
Internal bar code:AAGACTTCATGGGCATTTACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:551
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGTTTGCGCTTTTGGGG
Suggested primer 2:GACCTTGGGACTGGATGTGG