Insertion junction: CLIP2.011143_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.011143
Chromosome:chromosome 6
Location:2522388
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g269350 (1 of 1) K13211 - GC-rich sequence DNA-binding factor (GCFC) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCGCGCCCCGCCCGCGTCCGGCTGCCGCGCCTGCTGCTCCAAGCCCCG
Internal bar code:GGGGCGTCGATGTGCCGGCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:844
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATGGAACTCTCGCTTGT
Suggested primer 2:GAGGGTTTGCAGCAAAGTCG