| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011150 |
| Chromosome: | chromosome 9 |
| Location: | 3272642 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394991 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTCCTGGCTCGCCCCTCCGCCGCCCGACAGGTGGCTGTGCCGGGCTA |
| Internal bar code: | TAGGTTTTTTGGGCGGGCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 4.54545 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCGCAATCTTATTGACA |
| Suggested primer 2: | CCGCATTGACCCAAGTACCT |