Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.011151 |
Chromosome: | chromosome 3 |
Location: | 629912 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g145547 | CBR1 | (1 of 3) K00326 - cytochrome-b5 reductase (E1.6.2.2); NADH-cytochrome b5 reductase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTGGCAAACCCATGAAAGCGTAGCGGCGTGCGTTACGTGCCGCACGC |
Internal bar code: | ACATGGAAGTGGGACGTTGATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2583 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 86 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTAGGGCAGCTTGGTATCGG |
Suggested primer 2: | GAGAGGAAGTGGCTGCACAT |