Insertion junction: CLIP2.011160_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.011160
Chromosome:chromosome 16
Location:6539082
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g674500 ABCA4 ABC transporter 4; (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGGGGGCTCGAGCACTGTTGGTTGCACGCGCGCTGTGACGCTACCAGA
Internal bar code:CCCATGCCTGTTTTGCGGTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:435
LEAP-Seq percent confirming:78.5714
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTCTCCCCACACACCTA
Suggested primer 2:CAACCTGCACAGTGGAATGC