| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011163 |
| Chromosome: | chromosome 7 |
| Location: | 1730286 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325717 | (1 of 7) 2.4.99.18 - Dolichyl-diphosphooligosaccharide--protein glycotransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGGTGCCCAGCGTCTCCCTTGCAGCTCCGCAGCGCCGCCGTTGCTTT |
| Internal bar code: | GATTAGGAACTACAGAAATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1539 |
| LEAP-Seq percent confirming: | 35.2941 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGATAGCGTCCCACTAC |
| Suggested primer 2: | GTTATGTTGCGACGACACGG |