| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011178 |
| Chromosome: | chromosome 9 |
| Location: | 3682127 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397216 | (1 of 3) K01832 - thromboxane-A synthase (TBXAS1, CYP5A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGCAACCAGCCATGGGTGTGCGTGGCAGACCCCGACCTCATCAGGC |
| Internal bar code: | TCGCAGATAGGTCGAGTGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3902 |
| LEAP-Seq percent confirming: | 98.2143 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTCCCTTGCGCTCATTA |
| Suggested primer 2: | GATCGAGCGCTACCTGAGTC |