| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.011200 |
| Chromosome: | chromosome 10 |
| Location: | 2185279 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433550 | DNL4,LIG4,LIG2 | (1 of 21) IPR001357 - BRCT domain; DNA ligase 4 | intron |
| Cre10.g801121 | (1 of 1) PTHR10459:SF7 - DNA LIGASE 4 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTTACTTGTGCTTCCTAACACGATCTAGCGGCTGCTACTGCAGGTCAC |
| Internal bar code: | TGGTTAGGCGTGTAGATCTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2519 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCTTTGTACCTGGCTGCG |
| Suggested primer 2: | CGGCTGTAGCTCAAGGAACA |