| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.011207 |
| Chromosome: | chromosome 1 |
| Location: | 4216567 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028450 | (1 of 1) PTHR30544//PTHR30544:SF2 - 23S RRNA METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACACACACACACACACACACACACACACACACACACACACACAC |
| Internal bar code: | GGAGTTTGACATAAGGGTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1391 |
| LEAP-Seq percent confirming: | 77.1429 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAAGTTGCAGCCCATAT |
| Suggested primer 2: | CAACCTCATCCCCTGGAACC |