| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.011207 |
| Chromosome: | chromosome 1 |
| Location: | 4216589 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028450 | (1 of 1) PTHR30544//PTHR30544:SF2 - 23S RRNA METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGAATGCATGTGCGTGTGCGACCCTCCCTCCTAAGCGGTTACGCGCA |
| Internal bar code: | CAGCTCGCGTAGTAAGATTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCTCATCCCCTGGAACC |
| Suggested primer 2: | AGCCAAGTTGCAGCCCATAT |