Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.011230 |
Chromosome: | chromosome 6 |
Location: | 6342793 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g293051 | AMT1C,AMT3 | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCCAACGCCTTCATCGGCGACCACAACTTCGCGCTGTCGTACACCAC |
Internal bar code: | AGCCCATTTTATAATTGGCGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 763 |
LEAP-Seq percent confirming: | 72.0 |
LEAP-Seq n confirming: | 18 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGAACAAGGTCAGATGGGG |
Suggested primer 2: | TACAATTTGTGAGGGCGGCT |