| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.011242 |
| Chromosome: | chromosome 16 |
| Location: | 5489875 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801970 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGATCCTGCAGGAAGGCCACCCACGCCGCTGTGATGCAGCGCCCGGC |
| Internal bar code: | TTGCCAATTAGAGAGTGAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGTTTTTGGGTGGACG |
| Suggested primer 2: | GCACGAGTCAGATGGTTCCA |