| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.011243 |
| Chromosome: | chromosome 12 |
| Location: | 1829280 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512450 | (1 of 12) PF04749 - PLAC8 family (PLAC8) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTGTGCGGAGATGGCGGCCAACTGCTGCTACCCGTGCACGCTGGTCC |
| Internal bar code: | GCTCGGTTTGTGCGCCCGCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3021 |
| LEAP-Seq percent confirming: | 81.5789 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACAGTTTTGCACAGGC |
| Suggested primer 2: | ATGTGGAGGTGAGCTTTCGG |