Insertion junction: CLIP2.011283_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.011283
Chromosome:chromosome 3
Location:4116047
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g172550 PRM1,PRMT1 (1 of 2) K11434 - protein arginine N-methyltransferase 1 (PRMT1); Protein-/Histone-arginine N-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCGACGACCGCGTCACCATCATCAAGGGCAAGGTGGAGGAGGTGAGGC
Internal bar code:CACTTAGAAATTGGTATATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1295
LEAP-Seq percent confirming:96.7213
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCTGCTGTTGCCAAACCC
Suggested primer 2:CCGCCATGCTGTCATTTAGC