| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.011291 |
| Chromosome: | chromosome 16 |
| Location: | 1626281 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654250 | THB6 | Truncated hemoglobin; (1 of 2) PTHR10681:SF17 - PEROXIREDOXIN-6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGGACACCTTGCGAGGCATGCGGCGGCCCGAGGGCGTCCGCTCCGTG |
| Internal bar code: | TACAGGTGAGCATTTTGGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1985 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 128 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 176 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGCTGACTTTGTTTGACG |
| Suggested primer 2: | GCTCCCCTTCCTTCCCTTTC |