Insertion junction: CLIP2.011293_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.011293
Chromosome:chromosome 10
Location:950080
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g424550 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGTTCGCGGCGCGCACCTCGCTGGACATGCTGGACCTGGACGCCAAG
Internal bar code:TTGGTCTTAACCTTTATCAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1162
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CACGATGACGACGTTGTTGG
Suggested primer 2:ATGTGCTCTGGCCTTCTGTC