Insertion junction: CLIP2.011299_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.011299
Chromosome:chromosome 6
Location:1695785
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g262400 (1 of 1) K14676 - lysophospholipid hydrolase (NTE, NRE) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGCACAACGGTGCCAGCCCTTTTCCGAATTCATCGCCCCGGTGAGCTC
Internal bar code:TGCCTCCAGATGACGGGGTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:3399
LEAP-Seq percent confirming:98.2759
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:GTGACGTTGTTTTGGCCCTC
Suggested primer 2:ACACACATCTCCTTGCCCAG