Insertion junction: CLIP2.011301_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.011301
Chromosome:chromosome 10
Location:4240738
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g449650 (1 of 4) IPR001810//IPR020683 - F-box domain // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGACCAACTGAGGCTTGCTTTCTGACGCGCATCTGTCGACGTTTGCGC
Internal bar code:TCCATGGTCGTACGCGATCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:188
LEAP-Seq percent confirming:18.1818
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CATGACCCACTCCAACACGA
Suggested primer 2:CGAAATCCACGTGTGTGCTG