Insertion junction: CLIP2.011312_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.011312
Chromosome:chromosome 3
Location:6036057
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g189950 HOP1,HOP HSP70-HSP90 organizing protein; (1 of 1) K09553 - stress-induced-phosphoprotein 1 (STIP1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAATGCACTTGTCCGCGGCCTGGGGCGCGTCGGGCAGACACACAACGC
Internal bar code:GGATACTCCACGGGCGTTACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2849
LEAP-Seq percent confirming:82.1429
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:AAAAGGGCAACACCGCATTC
Suggested primer 2:TCGCCATGGGAAATCAACGA