Insertion junction: CLIP2.011322_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.011322
Chromosome:chromosome 12
Location:5234918
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g527500 (1 of 1) K04508 - transducin (beta)-like 1 (TBL1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCAGCTTGTGCAGGCAGCGCCCCACCTCCACGTCCCACAGCCGCACCGT
Internal bar code:GTGGTAGATCCCGGGTATTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4539
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:69
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTTCGCGCTCAAGTGGAA
Suggested primer 2:CCACACGCGAACAATGTGTT