| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011325 |
| Chromosome: | chromosome 12 |
| Location: | 1191040 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490451 | (1 of 16) IPR011044 - Quinoprotein amine dehydrogenase, beta chain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTTACAACCCCCTCAAGTCAGACCCCGCCTCACTCGCCTCGCTGCCA |
| Internal bar code: | TTGTAAATGTCCGGCACCTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2922 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 123 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 126 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAAGAATCGCATTCCCAG |
| Suggested primer 2: | CCCGCCCTCATGTTACTTGT |