| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.011361 |
| Chromosome: | chromosome 16 |
| Location: | 5191270 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684850 | TMG7 | (1 of 3) 2.1.1.33 - tRNA (guanine(46)-N(7))-methyltransferase / tRNA guanine 7-methyltransferase; tRNA (guanine-N7)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGCAGGCCAACGTGGTCGTGTCAGCGGCCGCCATTCTGGGCTCCTAC |
| Internal bar code: | AACTCAGCCTTACTCTGTCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1790 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGCTGTGTCTTCGAAG |
| Suggested primer 2: | CGAAGCAGGATCCACGCTAT |