| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011366 |
| Chromosome: | chromosome 10 |
| Location: | 4171699 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449020 | (1 of 1) 2.6.1.57//2.6.1.7 - Aromatic-amino-acid transaminase // Kynurenine--oxoglutarate transaminase / Kynurenine--oxoglutarate aminotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAGCTGAAGAAGGCGGCGCGGACACGGGCTCGGGCTGGAGTTGCAGC |
| Internal bar code: | AAAGTGACAAGAAGCCTAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1257 |
| LEAP-Seq percent confirming: | 88.4615 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTTAGGTGTGCGAAAGA |
| Suggested primer 2: | ACCAGACATGCCTAGCACAC |