Insertion junction: CLIP2.011393_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.011393
Chromosome:chromosome 16
Location:3719698
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g681657 (1 of 1) PF01344//PF13415//PF13418 - Kelch motif (Kelch_1) // Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCATCTGACAGCGCGCTGCGGCCGCGGCGGCCCCCGCCTAGCCACTG
Internal bar code:GCGCAGTCGTGTTTGGCGTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3140
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGGCTACAGCTGTATCGG
Suggested primer 2:CCTCAGGCATGCAACCAAAC