| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.011417 |
| Chromosome: | chromosome 10 |
| Location: | 6633633 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466150 | (1 of 8) PF00560 - Leucine Rich Repeat (LRR_1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTCGCTTTCTCCTTCTATAGGCTCTCGGCGAGAAAATGACCTGTTTCG |
| Internal bar code: | GAACGAGCAATATTCTGGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1389 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTATTGGGCAATGGTGGA |
| Suggested primer 2: | CGTGGATCCAAAGTCCAGCA |