| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011420 |
| Chromosome: | chromosome 6 |
| Location: | 5207927 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283800 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGAAGGGGCAAGTTGCTAAGGTTGGCAGCAGCAGGTGGATGGTTGGT |
| Internal bar code: | AATGCACGGGCCAGTTACCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 871 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCTCCATCTTACGTCGC |
| Suggested primer 2: | TTCATTAGCAGGGCTGTGGG |