| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.011432 |
| Chromosome: | chromosome 5 |
| Location: | 715041 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245350 | (1 of 1) IPR003582//IPR009011 - ShKT domain // Mannose-6-phosphate receptor binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCCAGCAGCCAGTCCTCTGCAAGGCGGGGGAAGGGGGGGGGCAAGGG |
| Internal bar code: | CTGTGTTTTTGATAGGATCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 125 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACGCGTCTACTCTGCAA |
| Suggested primer 2: | CACAAGGAACACATTGCGCA |