| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.011438 |
| Chromosome: | chromosome 4 |
| Location: | 2442175 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221650 | TOP4 | (1 of 1) PTHR10169//PTHR10169:SF48 - DNA TOPOISOMERASE/GYRASE // DNA GYRASE SUBUNIT A, CHLOROPLASTIC/MITOCHONDRIAL-RELATED; Topoisomerase type IV | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTAGTCATACTTACCCGCGATAAGCCTGGAACCCCACGGGCGACCATT |
| Internal bar code: | CTCAACGCTGCGTCCTTATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1894 |
| LEAP-Seq percent confirming: | 67.5676 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCTCCACTTCAACACCT |
| Suggested primer 2: | CGCAAAGACAGTGTGGTGTG |