Insertion junction: CLIP2.011456_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.011456
Chromosome:chromosome 6
Location:5957009
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g290150 CGL65,RIMP1,RIMP Factor involved in 30S ribosomal subunit maturation; (1 of 1) PF02576 - Putative ribosome maturation factor RimP (DUF150) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCCCGTGCTCTTCTTCCCTTCAAACCGCCTCCCCCCGACCCCACCTCC
Internal bar code:ATTTCGGTAAGCTCTCATCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1886
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CCATCCTTCACCCCATGCTT
Suggested primer 2:GTGTTTTCACTGGTGGTCGC