| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.011459 |
| Chromosome: | chromosome 10 |
| Location: | 1450606 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428150 | (1 of 1) PTHR10566:SF58 - PROTEIN D2023.6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATCCAGAACCAAAAGGCCTCGCGGCAGCCGGCCGCCGCAGACCGCAC |
| Internal bar code: | ACTTCTCAAAATAAACCATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 765 |
| LEAP-Seq percent confirming: | 60.8696 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCTGCCCGCACTACAAA |
| Suggested primer 2: | TGTTTTAAGGCGCAACACCG |