Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.011483 |
Chromosome: | chromosome 1 |
Location: | 8146911 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g068012 | PPR5,FAP330 | (1 of 6) PF01535 - PPR repeat (PPR); PentatricoPeptide Repeat protein 5 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGGGGCTGGGAAGGACAAGTGGGAAGACGCAGGCCGTTGGTGAGGCG |
Internal bar code: | CTGGTCGATGAAAGGAATTGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1173 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 20 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCATCAGCTCCTCGTACGTC |
Suggested primer 2: | AGCTAGCCCTGGGACCTAAA |