Insertion junction: CLIP2.011573_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.011573
Chromosome:chromosome 1
Location:347127
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g002050 APT1 Acid phosphatase; (1 of 1) K01078 - acid phosphatase (E3.1.3.2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCCGCACTCCGCCCCCACTCCGCCCGTGCTCCTGCCGCGTCCGACTTG
Internal bar code:CAATGCATTAGATGTCCCATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:165
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTCAGGTGGGAGCATGA
Suggested primer 2:GCATTGCCGTTCACACACAT