Insertion junction: CLIP2.011576_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.011576
Chromosome:chromosome 1
Location:4588202
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g031650 CGLD12 Conserved in the Green Lineage and Diatoms; (1 of 2) PF05637 - galactosyl transferase GMA12/MNN10 family (Glyco_transf_34) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATAAAGTCTGGCCCGGAGGGCTGGTTCAAAGGGTCTCCCGCGGCCGCC
Internal bar code:TACTTCCGATATACCTGGCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2137
LEAP-Seq percent confirming:81.9672
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACTGCTCAGGATTGGCA
Suggested primer 2:TTTCACTCACGGGTCCATGG