Insertion junction: CLIP2.011595_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.011595
Chromosome:chromosome 2
Location:3710367
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g100500 CYG22 (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGCGAGCAGGCGGCGGATGGTGGCGGCGGCGAGGGCGGCGCGGAGGGT
Internal bar code:GGGTTTCATTATGCGTACAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1785
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACCCCAACACTCCTGAC
Suggested primer 2:GTTCACGATCTTCCGCATGC