Insertion junction: CLIP2.011598_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.011598
Chromosome:chromosome 8
Location:3827400
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g381483 SDR15 (1 of 239) IPR016024 - Armadillo-type fold; Short-chain dehydrogenase/reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAAAAAATGGAGAGGGGCGACGGACGGGCTATACGCCAGAACCAAGCG
Internal bar code:GTCAATGGATTGTAGTGCGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:215
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CATTTACCACCACCCCTCCC
Suggested primer 2:CCAAGATGGCAGGGCACTTA