Insertion junction: CLIP2.011606_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.011606
Chromosome:chromosome 10
Location:2764222
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g438250 LAT2 Latrunculin-B sensitive 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCGGGCTGTCCGGAGCGGGAACTGGTGGAGAGAGTAGAGAAGGGCGGC
Internal bar code:TGCAGATCAATCTGTACCTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1969
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGCAGGTACCACATCAGCTT
Suggested primer 2:ATGCGCTAGAGATCACGGTG