| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011625 |
| Chromosome: | chromosome 9 |
| Location: | 396261 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749797 | FAP281 | Flagellar Associated Coiled-Coil Protein 281 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGTGCCTGATGCCGGCCTGCGACTGCGACGGCCCGGTTCCAGCCCT |
| Internal bar code: | AAGCGACATCTTTCAAATTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTCCTCAGACAGCTTGG |
| Suggested primer 2: | AACACACCTACATGCTCGCA |