Insertion junction: CLIP2.011628_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.011628
Chromosome:chromosome 4
Location:2948762
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g224700 NRT1 (1 of 1) K14638 - solute carrier family 15 (peptide/histidine transporter), member 3/4 (SLC15A3_4, PHT); Nitrate transporter NRT1 family intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACGTCTCCTGCACGCCTCCTAATCCACGCCGCCGCCTCCACGCCACCTC
Internal bar code:ATCTAAATGTATATTTAGTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:175
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGATTGGGGGCACTAAGC
Suggested primer 2:CCACACTGTGTTTTCTGCGG