| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011640 |
| Chromosome: | chromosome 1 |
| Location: | 6613608 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCGGAGAGACTCAGGTGCCTTGTTTCCATGATGTGCCATTGATCCCCG |
| Internal bar code: | CTGTTTGTTATACCTATAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2625 |
| LEAP-Seq percent confirming: | 2.72727 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 110 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGGCATGAAGTCCGTTT |
| Suggested primer 2: | ATGTTTGCGGCCCCTTATCA |