| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.011651 |
| Chromosome: | chromosome 3 |
| Location: | 1116055 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148750 | CLH1 | Chlorophyllase I; (1 of 1) PTHR33428:SF2 - CHLOROPHYLLASE-1 | outside_mRNA |
| Cre03.g148800 | (1 of 1) 2.1.1.4 - Acetylserotonin O-methyltransferase / Hydroxyindole O-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTGCTGGCTACTGTGTGCATTCGAGTCAGGTCGGTGAGGGGTCTAGG |
| Internal bar code: | GTACCTTAGTAACTATGACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1711 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGCACTGCTGGGTGAAG |
| Suggested primer 2: | CCGCTGCTGGGATACGTAAT |