| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.011652 |
| Chromosome: | chromosome 10 |
| Location: | 719860 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422400 | (1 of 89) 3.6.4.13 - RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACACGGGTGTGACGCGGGTGCAGTGCAAGGTGGCGTGAAGGACGGG |
| Internal bar code: | TCTAGGCGGCTGTGGTCTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 345 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAGCTTGAAACGGCATG |
| Suggested primer 2: | GGCTTGCTAACACCTACCGT |