Insertion junction: CLIP2.011676_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.011676
Chromosome:chromosome 12
Location:3389093
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g498000 (1 of 100) IPR001810 - F-box domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCACGCCGCGGCCGCGCTTTCTGTTTGCACACGTAGGTGGTGAACCAG
Internal bar code:TCAATACAGTAAGGTACTTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:3111
LEAP-Seq percent confirming:98.0392
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAACCCTATGCGCATCAG
Suggested primer 2:TCAATGGTGGACGCTGAGAC