| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011706 |
| Chromosome: | chromosome 1 |
| Location: | 6247597 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044350 | (1 of 2) PTHR11319//PTHR11319:SF28 - G PROTEIN-COUPLED RECEPTOR-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGCATGGCGTGCAACACTGCTGGTGCTAGTCATGGGACCTGCCCTAA |
| Internal bar code: | CGTTGATGGACTAAGGCGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2000 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACCTGCAGGATATGGAT |
| Suggested primer 2: | AGGGAGGGTTACGGCATTTG |