Insertion junction: CLIP2.011714_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.011714
Chromosome:chromosome 9
Location:1078033
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g401900 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCAGAGAGGCACCAACCACCAAACCCTACGTCATACTCTCATTGCATC
Internal bar code:GTATAGGGAGACATGTCCGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1401
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCTCTACTCGCCATGT
Suggested primer 2:CACGCAAGAGCAACCTGTTC